Description of the Circuit

Circuit construction

Genetic Circuit
A Genetic circuit consists of genetic toggle switch coupled with repressilator. Toggle switch has two mutually repressing genes that encode the repressor for the opposite gene. Repressilator includes three genes that code the repressor of next gene in cyclic manner.

For the construction of genetic circuit of IPCS, bidirectional reaction has to be considered.

Genetic toggle switch consists of IPCS_1 and IPCS_2.

Repressilator consist of Lactose repressor (LacI, Tetracycline repressor (Tetr), Lamda repressor (Phage lamda). In circuit, IPCS_1 encodes for the repressor for the opposite gene IPCS_2 and IPCS_2 represses IPCS_1. In the circuit, it is considered that IPCS_1 and IPCS_2 mutually repress each other that reflect the bistable behavior of the genetic switch for the IPCS.

Repressilator genes acts in cyclic manner where LacI represses Tetr, Tetr represses LamdaR and LamdaR in turn represses LacI, repressilator tends to produces oscillatory behavior. Coupling between genetic toggle switch and repressilator is developed by LacI and IPCS_1 where LacI regulates the IPCS_1 and represses it.