Catalogue

Modules of the Circuit

Genetic toggle switch -a synthetic, bistable gene-regulatory network consist of two repressors and two constitutive repressible promoters. Each promoter is inhibited by the repressor that is transcribed by the opposing promoter. When each gene encodes a transcriptional repressor of the other and each repressor is blocked by a chemical input, the system can be switched between two stable states.

Promoter

This is an important component in the DNA sequence which recruits transcriptional machinery and lead to transcription of the downstream DNA sequence.

Repressilator

Three transcriptional repressor systems can be used to build an oscillating network. The repressilator consists of three genes connected in a feedback loop, such that each gene represses the next gene in the loop and is repressed by the previous gene. In addition, green fluorescent protein is used as a reporter so that the behavior of the network can be observed using fluorescence microscopy.

Ribosome Binding Site

A Ribosome Binding Site (RBS) is an RNA sequence found in mRNA to which ribosomes can bind and initiate translation

Protein Coding Sequence

Protein coding sequences are DNA sequences that are transcribed into mRNA and in which the corresponding mRNA molecules are translated into a polypeptide chain.